Journal: bioRxiv

Article Title: A Rare T-Cell Factor 4 Lineage-negative Epithelial Stem Cell Supports Wound Repair and APC-deletion-induced Colon Tumorigenesis

doi: 10.64898/2026.03.17.712502

Figure Lengend Snippet: (A-B) Confocal Images, cell number, and crypt location of Epi Tcf4 Lin- and MIX cells (scale bar, 50 μm). Yellow arrows mark unique cellular characteristics (C) EdU labeling and FACs analysis for cell viability (Ca-d) and proliferation (Ce-h) of Epi Tcf4 Lin-, MIX and Tcf4 and Cd2 Lin+ cell populations. (D-E) A representative example of morphological analysis of fluorescence of Epi Tcf4 Lin- and MIX cell populations combined with (D) fluorescent EdU+ and (E) differential interference contrast (Nomarski DIC; scale bar, 10 μm). (F-G) Characterization of Epi Tcf4 Lin- and MIX populations (F) by co-staining with antibodies to known cell markers DCLK1 (Tuft) and PYY (Enteroendocrine L-cells) or (G) by morphology (scale bar in (F) is 20 μm and in (G) is 10 μm). Note that the MIX population is where mGFP and mTOM are labeling membranes in the same cell but are not overlapping.

Article Snippet: All other lines have been backcrossed and maintained in the C57/BL6 background: Tcf4 Cre , Tcf4 CreNeo , Rosa mTmG , Apc LoxEx1-15 (JAX stock #009045; ( )), Apc LoxEx5 (KOMP; APC tm1c; Reporter removed with FlpE recombination , as animals with reporter have a phenotype in the absence of Cre, and no phenotype without reporter until conditional deletion), Cd2 iCre (JAX stock #008520; ( ; )).

Techniques: Labeling, Fluorescence, Staining